Abstract
We have identified dEset, the fly homolog of human SETDB1 and mouse ESET histone lysine methyltransferases (HKMTases) that methylates the lysine 9 residue of histone 3 (H3-K9) and negatively regulates transcription of target genes. By using spatio-temporal RNA interference we show that dEset is required at several stages of development coinciding with ecdysone pulses, possibly as a repressor of transcription of target genes. Several interacting partners, for example USP, spire, and cut up were identified in a yeast two-hybrid screen. The spatio-temporal expression profiles of dEset and its potential partners suggest that they may act together or even in a larger complex.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Amino Acid Sequence
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Animals
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Animals, Genetically Modified
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Body Patterning / genetics
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Drosophila / enzymology
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Drosophila / genetics
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Drosophila / growth & development*
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Drosophila Proteins / genetics
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Drosophila Proteins / physiology*
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Gene Expression Regulation, Developmental
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Histone-Lysine N-Methyltransferase / genetics
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Histone-Lysine N-Methyltransferase / physiology*
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Male
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Mice
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Models, Genetic
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Molecular Sequence Data
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Phenotype
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Protein Methyltransferases / genetics
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Protein Structure, Tertiary
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RNA Interference
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Two-Hybrid System Techniques
Substances
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Drosophila Proteins
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Protein Methyltransferases
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Histone-Lysine N-Methyltransferase
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SETDB1 protein, mouse
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egg protein, Drosophila