In this study; we investigated the prevalence and distribution of malaria in the city of Diyarbakir, retrospectively. The investigation was carried out according to age, gender and areas of settlement between 1999 and 2004. The records were obtained from the Health Directorship of the Ministry of Health in Diyarbakir. A total of 22,062 malaria cases (11,643 male, 10,419 female) had been reported during a period of five years. Compared to previous years, the prevalence of malaria had significantly decreased. However; since the geographic conditions in Diyarbakir are suitable for the reproduction of Anopheles spp. and malaria is still endemic in Diyarbakir, the fight against malaria should be continued.