A new method of defense response analysis using a transient expression system in rice protoplasts

Biosci Biotechnol Biochem. 2007 Feb;71(2):590-3. doi: 10.1271/bbb.60526. Epub 2007 Feb 7.

Abstract

We established a new plant defense response assay using a transient expression system in rice protoplasts. The assay system sensitively detected defense induction by flagellin, which had previously been assigned to a specific elicitor. Our assay system provides a rapid and efficient way to dissect rice defense mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Flagellin / genetics
  • Flagellin / pharmacology
  • Gene Expression Regulation, Plant / genetics
  • Genes, Reporter / genetics
  • Luciferases / genetics
  • Luciferases / metabolism
  • Oryza / cytology
  • Oryza / physiology*
  • Plant Diseases*
  • Protoplasts / physiology*
  • Renilla / chemistry

Substances

  • Flagellin
  • Luciferases