Immune complexes of auto-antibodies against A beta 1-42 peptides patrol cerebrospinal fluid of non-Alzheimer's patients

Mol Psychiatry. 2007 Jun;12(6):601-10. doi: 10.1038/sj.mp.4001947. Epub 2007 Feb 6.

Abstract

The diagnostic potential of large A beta-peptide binding particles (LAPs) in the cerebrospinal fluid (CSF) of Alzheimer's dementia (AD) patients and non-AD controls (nAD) was evaluated. LAPs were detected by confocal spectroscopy in both groups with high inter-individual variation in number. Molecular imaging by confocal microscopy revealed that LAPs are heterogeneous superaggregates that could be subdivided morphologically into four main types (LAP 1-4). LAP-4 type, resembling a 'large chain of pearls', was detected in 42.1% of all nAD controls but it was virtually absent in AD patients. LAP-4 type could be selectively removed by protein A beads, a clear indication that it contained immunoglobulins in addition to beta-amyloid peptides (A beta 1-42). We observed a close correlation between LAPs and immunoglobulin G (IgG) concentration in CSF in controls but not in AD patients. Double labeling of LAPs with anti-A beta and anti-IgG antibodies confirmed that LAP-4 type consisted of A beta and IgG aggregates. Our results assign a central role to the immune system in regulating A beta1-42 homeostasis by clustering this peptide in immunocomplexes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / cerebrospinal fluid*
  • Alzheimer Disease / immunology
  • Amyloid beta-Peptides / cerebrospinal fluid
  • Amyloid beta-Peptides / immunology*
  • Antigen-Antibody Complex / cerebrospinal fluid*
  • Autoantibodies / cerebrospinal fluid*
  • Autoantibodies / classification
  • Biomarkers / cerebrospinal fluid
  • Case-Control Studies
  • Central Nervous System / immunology
  • Dementia / cerebrospinal fluid*
  • Dementia / classification
  • Dementia / immunology
  • Humans
  • Immunoglobulin G / cerebrospinal fluid*
  • Immunoglobulin G / immunology
  • Peptide Fragments / cerebrospinal fluid
  • Peptide Fragments / immunology*
  • Reference Values

Substances

  • Amyloid beta-Peptides
  • Antigen-Antibody Complex
  • Autoantibodies
  • Biomarkers
  • Immunoglobulin G
  • Peptide Fragments
  • amyloid beta-protein (1-42)