Tyrosine phosphorylation and lipid raft association of pseudorabies virus glycoprotein E during antibody-mediated capping

Virology. 2007 May 25;362(1):60-6. doi: 10.1016/j.virol.2006.12.018. Epub 2007 Jan 22.

Abstract

In specific cell types infected with the alphaherpesviruses herpes simplex virus and pseudorabies virus (PRV), addition of virus-specific antibodies results in redistribution of cell-surface-anchored viral proteins. This redistribution is triggered by the viral protein gE and consists of the directional movement of the antibody-antigen complexes to one pole of the cell. This viral capping process has been associated with increased antibody-resistant virus spread and strongly resembles immunoreceptor capping, a process that is crucial in activation of different immune cells (e.g. capping of Fcgamma-receptors, B and T cell receptors). Here, we report that the PRV gE-mediated viral capping process results in increased Src kinase-mediated tyrosine phosphorylation of the cytoplasmic domain of gE and that a fraction of gE associates with lipid rafts, all very reminiscent of immunoreceptor capping. These results provide evidence that gE-mediated capping is a viral mimicry of immunoreceptor capping.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Animals
  • Antibodies, Viral / metabolism*
  • Cell Line
  • Herpesvirus 1, Suid / immunology
  • Herpesvirus 1, Suid / metabolism*
  • Immunologic Capping
  • Membrane Microdomains / chemistry*
  • Microscopy, Confocal
  • Phosphorylation
  • Protein Structure, Tertiary
  • Swine
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / immunology
  • Viral Envelope Proteins / metabolism*
  • src-Family Kinases / metabolism

Substances

  • Antibodies, Viral
  • Viral Envelope Proteins
  • glycoprotein E, Suid herpesvirus 1
  • src-Family Kinases