Gas-related impact of pneumoperitoneum on systemic wound healing

Langenbecks Arch Surg. 2008 Jan;393(1):75-80. doi: 10.1007/s00423-006-0136-3. Epub 2007 Jan 13.

Abstract

Introduction: The aim of the present study was to investigate the gas-dependent effects of pneumoperitoneum on wound healing distant from the abdomen.

Materials and methods: Dorsal skin incisions were performed in 54 male Sprague-Dawley rats. A CO(2) or a helium pneumoperitoneum of 3 mmHg was maintained before, with an overall duration of 30 min (each n = 18). Rats in the control group received laparotomy only (n = 18). Animals were killed after 5 and 10 days. The infiltration of macrophages (CD 68), expression of matrix metalloproteinase 13 (MMP-13) and collagen I/III ratios were analysed in the dorsal skin wounds.

Results: Both after 5 and 10 days, infiltration of macrophages and expression of MMP-13 were greatest after helium pneumoperitoneum. After 5 days, collagen I/III ratio was significantly increased in the helium group. After 10 days, collagen I/III ratio was lowest in the CO(2) group.

Conclusion: Our results suggest beneficial effects on systemic wound healing for helium pneumoperitoneum as compared to CO(2).

MeSH terms

  • Animals
  • Antigens, CD / analysis
  • Antigens, Differentiation, Myelomonocytic / analysis
  • Carbon Dioxide*
  • Collagen Type I / analysis
  • Collagen Type III / analysis
  • Dermatologic Surgical Procedures*
  • Helium*
  • Immunoenzyme Techniques
  • Macrophage Activation / physiology
  • Macrophages / pathology
  • Male
  • Matrix Metalloproteinase 13 / analysis
  • Pneumoperitoneum, Artificial*
  • Rats
  • Rats, Sprague-Dawley
  • Skin / pathology*
  • Wound Healing / physiology*

Substances

  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD68 protein, rat
  • Collagen Type I
  • Collagen Type III
  • Carbon Dioxide
  • Helium
  • Matrix Metalloproteinase 13