Monitoring survival and function of transfused platelets in Bernard-Soulier syndrome by flow cytometry and a cone and plate(let) analyzer (Impact-R)

Transfusion. 2007 Jan;47(1):103-6. doi: 10.1111/j.1537-2995.2007.01070.x.

Abstract

Background: Bernard-Soulier syndrome (BSS) patients may repeatedly require transfusion of platelets (PLTs). The hemostatic competence of transfused PLTs requires monitoring.

Study design and methods: Flow cytometry and a cone and plate(let) analyzer (Impact-R, DiaMed) were used to monitor survival and function of transfused PLTs in a 7-year-old girl with BSS undergoing surgery. Flow cytometry was applied to differentiate autologous PLTs from transfused PLTs by staining for CD42b. The Impact, which measures PLT adhesion and aggregation in response to high shear stress, was used to evaluate PLT function.

Results: Transfused PLTs were detectable by flow cytometry for 1 week after transfusion. While the patient's PLTs did not respond to high shear stress before transfusion, a normal response was documented by the Impact on the day after transfusion and 1 week thereafter.

Conclusion: Transfused PLTs were detectable by flow cytometry, and their functional activity was demonstrated by the Impact.

Publication types

  • Case Reports

MeSH terms

  • Bernard-Soulier Syndrome / complications
  • Bernard-Soulier Syndrome / physiopathology*
  • Bernard-Soulier Syndrome / therapy*
  • Blood Platelets* / immunology
  • Cell Survival
  • Child
  • Defibrillators, Implantable
  • Female
  • Flow Cytometry*
  • Humans
  • Long QT Syndrome / complications
  • Long QT Syndrome / surgery
  • Platelet Adhesiveness
  • Platelet Aggregation
  • Platelet Function Tests*
  • Platelet Glycoprotein GPIb-IX Complex / metabolism
  • Platelet Transfusion*
  • Preoperative Care
  • Time Factors

Substances

  • Platelet Glycoprotein GPIb-IX Complex