Objective: The feasibility of Papaver somniferum L. cultivars identification was explored by TD-RAPD technique.
Method: Genomic DNA was extracted by improved CTAB method. One sample of species from Papaver somniferum L in xishuangbanna area. was studied by using TD-RAPD method.
Result: We established an optimal method of extracting genomic DNA. Six primers were picked out from 10 primers.
Conclusion: TD-RAPD could be applied to researches of molecular marker of Papaver somniferum L. TD-RAPD technique provide a method to constitute DNA database of Papaver somniferum L. and conclude the source of opium poppy.