Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene

Rippei Hayashi; Takuya Ueda; Mary A Farwell; Nono Takeuchi

doi:10.1016/j.mito.2006.10.005

Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene

Mitochondrion. 2007 May;7(3):195-203. doi: 10.1016/j.mito.2006.10.005. Epub 2006 Nov 7.

Authors

Rippei Hayashi¹, Takuya Ueda, Mary A Farwell, Nono Takeuchi

Affiliation

¹ Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Building FSB-401, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture 277-8562, Japan.

PMID: 17161026
DOI: 10.1016/j.mito.2006.10.005

Abstract

We studied the transcriptional regulation of the human mitochondrial translation initiation factor 2 (IF2mt) gene. The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes. Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2alpha/beta binds to the IF2mt promoter. Reporter assays, where HEK293T cells were co-transfected with an NRF-2alpha/beta-expressing vector and/or an IF2mt promoter reporter vector, revealed that NRF-2 trans-activates the IF2mt promoter. NRF-2 sites were also found in the promoters of several other mitochondrial translation factors, which suggests NRF-2 may play a key role in the regulation of mitochondrial protein synthesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
DNA Primers
GA-Binding Protein Transcription Factor / physiology*
Gene Expression Regulation*
Humans
Kidney
Molecular Sequence Data
Plasmids
Prokaryotic Initiation Factor-2 / genetics
Prokaryotic Initiation Factor-2 / metabolism*
Transcription, Genetic*
Transfection

Substances

DNA Primers
GA-Binding Protein Transcription Factor
Prokaryotic Initiation Factor-2