Rapid pulsed-field gel electrophoresis protocol for subtyping of Streptococcus suis serotype 2

Cindy K Y Luey; Yiu Wai Chu; Terence K M Cheung; Catherine C P Law; Man Yu Chu; Danny T L Cheung; Kai Man Kam

doi:10.1016/j.mimet.2006.10.010

Rapid pulsed-field gel electrophoresis protocol for subtyping of Streptococcus suis serotype 2

J Microbiol Methods. 2007 Mar;68(3):648-50. doi: 10.1016/j.mimet.2006.10.010. Epub 2006 Dec 8.

Authors

Cindy K Y Luey¹, Yiu Wai Chu, Terence K M Cheung, Catherine C P Law, Man Yu Chu, Danny T L Cheung, Kai Man Kam

Affiliation

¹ Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong SAR, China.

PMID: 17157941
DOI: 10.1016/j.mimet.2006.10.010

Abstract

A rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping of Streptococcus suis serotype 2 was developed and evaluated using 27 clinical isolates from 22 epidemiologically unrelated patients. Results were matched against antibiogram, virulence genotyping and multi locus sequence typing (MLST). PFGE appeared to be the most discriminatory with numerical index of discrimination (D) equal to 0.87.

Publication types

Evaluation Study

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Bacterial Typing Techniques
DNA, Bacterial / analysis
Electrophoresis, Gel, Pulsed-Field / methods*
Humans
Microbial Sensitivity Tests
Serotyping
Streptococcus suis / classification*
Streptococcus suis / drug effects
Streptococcus suis / genetics
Streptococcus suis / pathogenicity
Time Factors
Virulence / genetics

Substances

Anti-Bacterial Agents
Bacterial Proteins
DNA, Bacterial