Abstract
To create a new experimental model of diabetes, we used the New Zealand Black (NZB) mouse as a potential model. NZB mice were immunized with B:9-23 insulin peptide in IFA and the viral mimic, poly(A:U). No diabetes was observed but blood glucose was significantly higher in the B:9-23 peptide group compared to controls. Insulin autoantibodies (IAA) were only induced in groups given the B:9-23 peptide. B:9-23 alone induced peri-insulitis. We demonstrate insulin autoimmunity in the NZB mouse using the insulin peptide B:9-23 and viral mimics. The reason for the protection from diabetes despite the presence of autoimmunity is currently not established.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / administration & dosage
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Adjuvants, Immunologic / pharmacology*
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Animals
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Autoimmunity / drug effects*
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Blood Glucose / analysis
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Diabetes Mellitus, Experimental / blood
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Diabetes Mellitus, Experimental / genetics*
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Diabetes Mellitus, Experimental / immunology*
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Diabetes Mellitus, Experimental / pathology
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Disease Models, Animal
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Female
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Follow-Up Studies
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Insulin / administration & dosage
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Insulin / chemistry
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Insulin / pharmacology*
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Islets of Langerhans / immunology
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Islets of Langerhans / pathology
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Mice
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Mice, Mutant Strains
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Peptide Fragments / administration & dosage
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Peptide Fragments / chemistry
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Peptide Fragments / pharmacology*
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Poly A-U / administration & dosage
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Poly A-U / pharmacology*
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Time Factors
Substances
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Adjuvants, Immunologic
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Blood Glucose
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Insulin
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Peptide Fragments
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Poly A-U