Enhanced insulin-like growth factor I gene expression in regenerating rat pancreas

Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6152-6. doi: 10.1073/pnas.88.14.6152.

Abstract

Insulin-like growth factor I (IGF-I) mRNA expression was studied after 90% partial pancreatectomy in the rat to determine whether IGF-I was associated with pancreatic regeneration. The level of IGF-I mRNA was maximally increased (4-fold above control value) 3 days after pancreatectomy, but thereafter gradually decreased, returning to control levels by 14 days after surgery. By in situ hybridization, IGF-I mRNA in both pancreatectomized and sham-operated rats was localized to capillary endothelial cells, indicating that this is the site of IGF-I expression in the normal rat pancreas. However, enhanced IGF-I mRNA expression was localized to focal areas of regeneration unique to pancreatectomized rats. In these areas, epithelial cells of proliferating ductules and individual connective tissue cells expressed IGF-I, suggesting that IGF-I may play an important role in the growth or differentiation of pancreatic tissue.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • DNA Probes
  • Gene Expression
  • Insulin-Like Growth Factor I / genetics*
  • Kinetics
  • Male
  • Nucleic Acid Hybridization
  • Pancreas / physiology*
  • Pancreatectomy
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Inbred Strains
  • Regeneration*
  • Sulfur Radioisotopes
  • Time Factors

Substances

  • DNA Probes
  • RNA, Messenger
  • Sulfur Radioisotopes
  • RNA
  • Insulin-Like Growth Factor I