Objective: To establish a miniCTTA multiplex set including short tandem repeat (STR) markers as CSF1PO, TH01, and TPOX and amelogenin gene, whose amplified fragments are shorter than 130 bp.
Methods: The length of the gene fragments amplified with fluorescence-labeled primers were analyzed by ABI3100 Genetic Analyzer for genotyping of 100 unrelated individuals, 10 genealogies and 30 highly degraded specimens.
Results: The genotypes derived form miniCTTA were consistent with the results by AmpFLSTR Identifiler kit.
Conclusion: MiniCTTA multiplex set is useful for personal identification and paternity test, especially applicable for degraded DNA sample.