Identification of type-specific linear epitopes in the glycoproteins gp46 and gp21 of human T-cell leukemia viruses type I and type II using synthetic peptides

Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5754-8. doi: 10.1073/pnas.88.13.5754.

Abstract

Synthetic peptides of 20-25 amino acids were employed in enzyme-linked immunosorbent assays to identify linear epitopes in the external glycoprotein gp46 and the transmembrane glycoprotein gp21 of human T-cell leukemia/lymphotropic viruses type I (HTLV-I) and II (HTLV-II). Ten linear epitopes were identified in the HTLV-I glycoproteins, seven in gp46 and three in gp21. Three major linear epitopes were identified in the gp46 of HTLV-II. Peptides representing linear epitopes of gp46 were found to be sensitive and specific for the detection of antibody and permit serological identification and differentiation of HTLV-I and HTLV-II infections.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antibody Specificity
  • Deltaretrovirus Antibodies / immunology
  • Deltaretrovirus Antigens / immunology*
  • Epitopes
  • Gene Products, env / immunology*
  • HTLV-I Infections / immunology
  • HTLV-II Infections / immunology
  • Human T-lymphotropic virus 1 / immunology*
  • Human T-lymphotropic virus 2 / immunology*
  • Humans
  • Molecular Sequence Data
  • Retroviridae Proteins, Oncogenic / immunology*
  • Species Specificity
  • env Gene Products, Human Immunodeficiency Virus

Substances

  • Deltaretrovirus Antibodies
  • Deltaretrovirus Antigens
  • Epitopes
  • Gene Products, env
  • Retroviridae Proteins, Oncogenic
  • env Gene Products, Human Immunodeficiency Virus
  • gp21 protein, Human T-lymphotropic virus 1
  • gp46 protein, Human T-cell leukemia virus type I
  • gp46 protein, Human immunodeficiency virus 2
  • human T-cell leukemia virus type-II protein gp21