Estimation of the chloramphenicol and cycloheximide inhibition of protein synthesis in brain cholinergic synaptosomes

Brain Res. 1991 Mar 15;543(2):351-3. doi: 10.1016/0006-8993(91)90049-2.

Abstract

Cholinergic synaptosomes have been prepared from sheep brain cortex by means of an immunoaffinity method using a specific anti-(Chol I) antiserum. The [14C]leucine incorporation into proteins of this preparation shows a low cycloheximide and a high chloramphenicol sensitivity. This fact suggests that the mitochondrial protein synthesis system is the only one present in this fraction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Chemistry / drug effects
  • Chloramphenicol / pharmacology*
  • Cycloheximide / pharmacology*
  • In Vitro Techniques
  • Leucine / metabolism
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Nerve Tissue Proteins / biosynthesis*
  • Parasympathetic Nervous System / drug effects
  • Parasympathetic Nervous System / metabolism*
  • RNA / metabolism
  • Sheep
  • Synaptosomes / drug effects
  • Synaptosomes / metabolism*

Substances

  • Nerve Tissue Proteins
  • RNA
  • Chloramphenicol
  • Cycloheximide
  • Leucine