Abstract
Monoclonal antibody-based immunoassays can detect specifically intact human chorionic gonadotropin (hCG), the free alpha-subunit and the free beta-subunit. The differential production of hCG and its free subunits is important in gestational trophoblastic disease. Using well-defined epitope-specific monoclonal antibodies in an immunoradiometric assay format, specific and sensitive assays have been developed. Serum levels of free beta-hCG were abnormally high in patients with gestational trophoblastic disease. In contrast, free alpha-hCG levels in serum were not increased.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal
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Chorionic Gonadotropin / blood*
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Chorionic Gonadotropin, beta Subunit, Human
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Immunoradiometric Assay / standards
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Peptide Fragments / blood*
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Pregnancy
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Sensitivity and Specificity
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Trophoblastic Neoplasms / blood*
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Trophoblastic Neoplasms / epidemiology
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Trophoblastic Neoplasms / genetics
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Uterine Neoplasms / blood*
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Uterine Neoplasms / epidemiology
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Uterine Neoplasms / genetics
Substances
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Antibodies, Monoclonal
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Chorionic Gonadotropin
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Chorionic Gonadotropin, beta Subunit, Human
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Peptide Fragments