Abstract
Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacteria / metabolism*
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Biological Assay / methods
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Electrochemistry / methods
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Glycoside Hydrolases / metabolism*
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Mannosides / metabolism
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Nitrophenols / analysis
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Nitrophenols / metabolism
Substances
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Mannosides
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Nitrophenols
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4-nitrophenyl-beta-D-mannopyranoside
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Glycoside Hydrolases