Abstract
Endothelial leukocyte adhesion molecule-1 (ELAM-1) is an endothelial cell adhesion molecule that allows myeloid cells to attach to the walls of blood vessels adjacent to sites of inflammation. ELAM-1 recognizes the sialyl-Lewis X (sialyl-Lex) determinant, NeuAc alpha 2-3Gal beta 1-4(Fuc alpha 1-3)GlcNAc-, a granulocyte carbohydrate also found on the surface of some tumor cell lines. Binding of myeloid cells to soluble ELAM-1 is inhibited by a monoclonal antibody recognizing sialyl-Lex or by proteins bearing sialyl-Lex, some of which may participate in humoral regulation of myeloid cell adhesion. Stimulated granulocytes also release an inhibitor of ELAM-1 binding that can be selectively adsorbed by monoclonal antibody to sialyl-Lex.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amniotic Fluid / chemistry
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Antibodies, Monoclonal / pharmacology
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Carbohydrate Sequence
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Cell Adhesion / physiology
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Cell Adhesion Molecules / immunology
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Cell Adhesion Molecules / metabolism*
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Cell Membrane / metabolism
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E-Selectin
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Endothelium, Vascular / metabolism
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Fucose / metabolism
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Fucosyltransferases / metabolism
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Granulocytes / metabolism*
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Immunosorbent Techniques
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Interleukin-1 / pharmacology
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Interleukin-8 / pharmacology
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Lewis X Antigen / chemistry
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Lewis X Antigen / metabolism*
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Molecular Sequence Data
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Neoplasms / metabolism*
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Neuraminidase / metabolism
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Oligosaccharides / chemistry
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Orosomucoid / metabolism
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Sialyl Lewis X Antigen
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Tumor Cells, Cultured
Substances
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Antibodies, Monoclonal
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Cell Adhesion Molecules
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E-Selectin
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Interleukin-1
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Interleukin-8
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Lewis X Antigen
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Oligosaccharides
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Orosomucoid
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Sialyl Lewis X Antigen
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Fucose
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Fucosyltransferases
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Neuraminidase