Lassa virus glycoproteins: antigenic and immunogenic properties of synthetic peptides to GP1

Arch Virol. 1990;115(1-2):133-7. doi: 10.1007/BF01310630.

Abstract

Synthetic peptides corresponding to predicted Lassa virus GP1 glycoprotein B-epitopes were used to study the antigenicity and immunogenicity of the protein. ELISA results showed that guinea pig polyclonal anti-Lassa virus serum bound effectively to peptides corresponding to amino acid residues 119-133 and 164-176 of the GP1 protein. Essentially it did not react to a peptide corresponding to GP1 amino acid residues 234-256. Sera obtained against peptides representing amino acid residues 119-133 and 164-176 reacted with inactivated purified Lassa virus.

MeSH terms

  • Amino Acid Sequence
  • Antigens, Viral / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / immunology
  • Glycoproteins / immunology*
  • Immune Sera / immunology
  • Lassa virus / immunology*
  • Molecular Sequence Data
  • Peptides / chemical synthesis
  • Peptides / immunology*
  • Viral Envelope Proteins / immunology*

Substances

  • Antigens, Viral
  • Epitopes
  • Glycoproteins
  • Immune Sera
  • Peptides
  • Viral Envelope Proteins
  • glycoprotein gp1, lassa virus