Dual colour, microarray-based, analysis of 10,000 protease substrates

Chem Commun (Camb). 2006 Oct 14:(38):3984-6. doi: 10.1039/b609029j. Epub 2006 Sep 8.

Abstract

A 10,000 member PNA-encoded library of FRET based peptides was synthesised for global analysis of protease cleavage specificity; analysis was achieved using a DNA microarray and consumed minimal quantities of enzyme (60 pmole) and library (3.5 nmole).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chymopapain / metabolism*
  • Color
  • Fluorescence Resonance Energy Transfer
  • Oligonucleotide Array Sequence Analysis / methods
  • Peptide Hydrolases / metabolism*
  • Peptide Nucleic Acids / chemistry
  • Protein Array Analysis / methods*
  • Substrate Specificity

Substances

  • Peptide Nucleic Acids
  • Peptide Hydrolases
  • Chymopapain