The risk of porcine endogenous retrovirus (PERV) infection is one of the major barriers in clinical trials of pig-to-human xenotransplantation. Previous experiments showed that PERV could infect many types of human and nonhuman primate cells, but there is no reported evidence of in vivo infection. In this study, extracted genomic DNA from tissues of seventeen pigs was analyzed using specific sequence primers for gag, pol, and env. The results suggested that PERV exist in the genomes of all tissues. A subtype analysis indicated that PERV-A and PERV-B were in the tissue genome with no positive PERV-C. A greater understanding of the properties of PERV in different pig tissues is necessary to evaluate the risk posed by PERV.