FTMS and TOF/TOF mass spectrometry in concert: identifying peptides with high reliability using matrix prespotted MALDI target plates

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Feb 15;847(1):62-4. doi: 10.1016/j.jchromb.2006.08.031. Epub 2006 Sep 11.

Abstract

In this paper we describe a combination of the mass spectrometric techniques MALDI-TOF/TOF and MALDI-FTMS to identify proteins in complex samples using prespotted MALDI target plates. By this procedure accurate FTMS mass measurements and TOF/TOF data are obtained from the same spot. We have found that this combination of techniques leads to more reliable identification of peptides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Proteins / analysis*
  • Blood Proteins / chemistry
  • Chromatography, Liquid
  • Humans
  • Mass Spectrometry / methods*
  • Molecular Weight
  • Peptide Mapping / methods*
  • Peptides / blood*
  • Peptides / chemistry
  • Proteome / analysis
  • Proteome / chemistry
  • Reproducibility of Results
  • Serum / chemistry
  • Spectrometry, Mass, Electrospray Ionization
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Spectroscopy, Fourier Transform Infrared

Substances

  • Blood Proteins
  • Peptides
  • Proteome