Real-time quantitative allele discrimination assay using 3' locked nucleic acid primers for detection of low-percentage mosaic mutations

Anal Biochem. 2006 Dec 1;359(1):144-6. doi: 10.1016/j.ab.2006.07.039. Epub 2006 Aug 14.

Authors

Ophélia Maertens¹, Eric Legius, Frank Speleman, Ludwine Messiaen, Jo Vandesompele

Affiliation

¹ Center for Medical Genetics, Ghent University Hospital, B-9000 Ghent, Belgium.

PMID: 16962063
DOI: 10.1016/j.ab.2006.07.039

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles*
Base Pair Mismatch / genetics
Base Pairing
Biological Assay
DNA Mutational Analysis* / methods
DNA Primers / genetics
Nucleic Acid Hybridization / genetics
Nucleic Acid Hybridization / methods
Oligonucleotide Probes / chemistry
Oligonucleotide Probes / genetics
Oligonucleotides
Oligonucleotides, Antisense / chemistry*
Oligonucleotides, Antisense / genetics*
Point Mutation / genetics*
Polymerase Chain Reaction / methods*
Polymerase Chain Reaction / standards
Polymerase Chain Reaction / statistics & numerical data
Polymorphism, Single Nucleotide / genetics*
Sensitivity and Specificity

Substances

DNA Primers
Oligonucleotide Probes
Oligonucleotides
Oligonucleotides, Antisense
locked nucleic acid