Identifying microRNA regulators of cell death in Drosophila

Methods Mol Biol. 2006:342:229-40. doi: 10.1385/1-59745-123-1:229.

Abstract

Animal genomes contain on the order of at least hundreds of microRNAs (miRNAs). Although most remain uncharacterized, it is already clear that miRNAs regulate many biological processes. A number of Drosophila miRNAs have been identified as likely cell death regulators, but functions for most have simply not been explored. Here we describe a protocol for identifying miRNAs that can act as cell death regulators. We also describe a simple protocol for testing roles for mRNAs identified as candidate miRNA targets using computational or other approaches.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cell Death / physiology
  • Computational Biology / methods
  • Drosophila / genetics*
  • Gene Expression Regulation / physiology*
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism*

Substances

  • MicroRNAs