Human T cell receptor-gamma delta-expressing T-cell lines recognize MHC-controlled elements on autologous EBV-LCL that are not HLA-A, -B, -C, -DR, -DQ, or -DP

J Immunol. 1990 Jul 1;145(1):36-45.

Abstract

HLA-loss variants of an EBV-transformed B lymphoblastoid cell line (EBV-LCL) 721 were used to investigate whether human MHC molecules other than known class I or class II were involved in autologous T cell responses. Bulk lymphocyte cultures of purified T cells primed to an autologous variant EBV-LCL that fails to express HLA-class II and has reduced cell surface HLA-class I expression, and oligoclonal TCR-gamma delta-bearing lines derived from them, could lyse both this EBV-LCL and an independently derived, class II expressing autologous variant EBV-LCL that bears no HLA-A, -B, or -C, suggesting the presence of additional HLA-like restriction elements. Cold target inhibition of cytolysis mediated by these lines indicated that a shared or cross-reactive MHC controlled restriction element other than the known MHC determinants was retained by the EBV-LCL variants. Single-cell derived clones from these T cell lines which expressed only the TCR-gamma delta showed this same target cell specificity pattern, proving recognition of MHC-controlled determinants by autologous gamma delta T cells. Anti-gamma delta antibody could inhibit cytolysis by the gamma delta-expressing lines, suggesting that the TCR-gamma delta was involved in recognition of the EBV-LCL targets. Flow cytometric analysis with separate HLA-reactive antibodies indicated that the restriction element for these cytolytic responses is a molecule serologically cross-reactive with HLA-B and -C Ag, yet is a determinant that cannot be HLA-A, -B, -C, -DR, -DQ or -DP.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, CD / analysis
  • Antigens, Surface / immunology
  • B-Lymphocytes / immunology*
  • Cytotoxicity, Immunologic
  • Epitopes
  • Flow Cytometry
  • HLA-D Antigens / immunology*
  • Histocompatibility Antigens Class I / immunology*
  • Humans
  • Immunity, Cellular
  • In Vitro Techniques
  • Lymphocyte Cooperation
  • Major Histocompatibility Complex*
  • Receptors, Antigen, T-Cell / classification
  • Receptors, Antigen, T-Cell / immunology*
  • T-Lymphocytes / immunology*
  • Tumor Cells, Cultured

Substances

  • Antigens, CD
  • Antigens, Surface
  • Epitopes
  • HLA-D Antigens
  • Histocompatibility Antigens Class I
  • Receptors, Antigen, T-Cell