Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis

FEMS Microbiol Lett. 2006 Sep;262(2):210-5. doi: 10.1111/j.1574-6968.2006.00390.x.

Abstract

A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV-Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA-85B or DNA-(85B-Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung-1 in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette-Guérin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyltransferases / genetics
  • Acyltransferases / immunology*
  • Animals
  • Antibodies, Bacterial / blood*
  • Antibodies, Bacterial / immunology
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology*
  • Female
  • Gene Products, tat / genetics
  • Gene Products, tat / immunology*
  • Genes, tat
  • HIV-1 / genetics
  • HIV-1 / immunology
  • Immunoglobulin G / biosynthesis
  • Interferon-gamma / biosynthesis
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Mycobacterium bovis / immunology
  • Mycobacterium tuberculosis / growth & development
  • Mycobacterium tuberculosis / immunology*
  • NIH 3T3 Cells
  • Plasmids
  • Promoter Regions, Genetic
  • Specific Pathogen-Free Organisms
  • Spleen / cytology
  • Transfection
  • Tuberculosis / immunology
  • Tuberculosis / microbiology
  • Tuberculosis / prevention & control*
  • Tuberculosis Vaccines / immunology*
  • Vaccines, DNA / immunology*
  • tat Gene Products, Human Immunodeficiency Virus

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Proteins
  • Gene Products, tat
  • Immunoglobulin G
  • Tuberculosis Vaccines
  • Vaccines, DNA
  • tat Gene Products, Human Immunodeficiency Virus
  • Interferon-gamma
  • Acyltransferases
  • antigen 85B, Mycobacterium tuberculosis