Efficient and reproducible large-scale isolation of human CD4+ CD25+ regulatory T cells with potent suppressor activity

J Immunol Methods. 2006 Aug 31;315(1-2):27-36. doi: 10.1016/j.jim.2006.06.014. Epub 2006 Jul 21.

Abstract

CD4+ CD25+ regulatory T cells have been the subject of intense investigation and have been shown to modulate immune responses in the settings of autoimmunity, cancer and transplantation. The assessment and optimization of purification schemes for specific cellular subtypes such as CD4+ CD25+ regulatory T cells is a critical consideration in developing cell-based therapies in the clinical setting. In the following studies, different strategies for magnetic isolation are compared and the parameters which affect the overall potency of purified human CD4+ CD25+ regulatory T cells are discussed. The data demonstrate that large-scale magnetic isolation can be used to efficiently and reproducibly purify human CD4+ CD25+ regulatory T cells capable of modulating alloreactive T cell responses. The ability to rapidly purify the desired cells from peripheral blood suggests that magnetic isolation may be a suitable alternative to cell sorting for clinical settings, where large numbers of CD4+ CD25+ regulatory T cells may be necessary.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Forkhead Transcription Factors / metabolism
  • Humans
  • Immunomagnetic Separation / methods*
  • Immunomagnetic Separation / standards
  • Interleukin-2 Receptor alpha Subunit / metabolism*
  • Reproducibility of Results
  • T-Lymphocytes, Regulatory / physiology*

Substances

  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • Interleukin-2 Receptor alpha Subunit