BDNF regulation in the rat dorsal vagal complex during stress-induced anorexia

Brain Res. 2006 Aug 30;1107(1):52-7. doi: 10.1016/j.brainres.2006.05.099. Epub 2006 Jul 18.

Abstract

The dorsal vagal complex (DVC) is the satiety reflex-integrating center of adult mammals. Immobilization stress (IS) is known to elicit anorexia and to up-regulate BDNF expression in adult rat forebrain; intra-DVC delivery of BDNF was shown to elicit anorexia. Therefore, we addressed here whether IS would increase BDNF signaling in rat DVC by using PCR and western-blot on microdissected tissue extracts. Significant variations of BDNF expression in DVC after IS include exon V mRNA increase at 3 h, decreases of both protein and exon III mRNA at 24 h, and exon I mRNA decrease at 72 h. At the receptor level, IS elicited a highly significant induction of both full-length and truncated-1 TrkB mRNAs at 24 h after IS. In vivo recruitment of BDNF signaling in DVC during stress thus differs from hypothalamus, the relevance of which to anorexia is discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Anorexia / etiology
  • Anorexia / metabolism*
  • Body Weight / physiology
  • Brain Stem / metabolism*
  • Brain-Derived Neurotrophic Factor / genetics
  • Brain-Derived Neurotrophic Factor / metabolism*
  • Gene Expression Regulation / physiology*
  • Male
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Receptor, trkB / genetics
  • Receptor, trkB / metabolism
  • Restraint, Physical / methods
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Stress, Physiological / complications
  • Time Factors

Substances

  • Brain-Derived Neurotrophic Factor
  • RNA, Messenger
  • Receptor, trkB