Evaluation of the resazurin microtiter assay for rapid detection of ofloxacin resistance in M. tuberculosis

Int J Tuberc Lung Dis. 2006 Jul;10(7):808-11.

Abstract

Objective: To evaluate the performance of the colorimetric resazurin microtiter assay (REMA) method for the detection of ofloxacin resistance.

Methods: A panel of 120 multidrug-resistant Mycobacterium tuberculosis strains was tested blindly by the REMA method and compared with the results obtained using the BACTEC 460 method.

Result: A very good correlation was observed between the two methods.

Conclusion: The REMA method is simple, rapid and can be an inexpensive alternative procedure for the rapid detection of anti-tuberculosis drug resistance in laboratories with limited resources.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Drug Resistance, Microbial*
  • Microbial Sensitivity Tests
  • Mycobacterium tuberculosis / drug effects*
  • Ofloxacin / pharmacology*
  • Oxazines / chemistry*
  • Xanthenes / chemistry*

Substances

  • Anti-Bacterial Agents
  • Oxazines
  • Xanthenes
  • resazurin
  • Ofloxacin