Derivation and characterization of lentoid bodies and retinal pigment epithelial cells from monkey embryonic stem cells in vitro

Methods Mol Biol. 2006:330:417-29. doi: 10.1385/1-59745-036-7:417.

Abstract

For cell replacement therapies of retinal diseases, the most-needed cells are retinal pigment epithelial cells and photoreceptor cells; lens cells are needed for replacement of the cataract. Stromal cell-derived inducing activity induces the differentiation of mouse embryonic stem (ES) cells into neural cells, including midbrain tyrosine hydroxylase-positive dopaminergic neurons, and ocular cells; the method also works with primate ES cells. We describe the methods to induce retinal pigment epithelial and lens cells from monkey ES cells using stromal cell-derived inducing activity and show the characteristics of those cells in vitro and in vivo.

MeSH terms

  • Animals
  • Behavior, Animal / physiology
  • Cell Culture Techniques*
  • Cell Differentiation / physiology*
  • Cell Transplantation
  • Cells, Cultured
  • Embryo, Mammalian / cytology
  • Epithelial Cells / cytology
  • Epithelial Cells / physiology*
  • Macaca fascicularis
  • Mice
  • Pigment Epithelium of Eye / cytology*
  • Rats
  • Stem Cells / cytology
  • Stem Cells / physiology*