Abstract
The human chloride intracellular channel protein CLIC4 has been crystallized by the hanging-drop vapour-diffusion technique using trisodium citrate as the precipitant. The best crystals were obtained by the microseeding method. The crystals diffracted to 2.2 A resolution and were found to belong to space group P121, with unit-cell parameters a = 73.19, b =86.05, c = 73.38 A, beta = 112.99 degrees and three molecule per asymmetric unit.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chloride Channels / chemistry*
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Chloride Channels / genetics
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Chloride Channels / isolation & purification*
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Chloride Channels / metabolism
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Crystallization
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Crystallography, X-Ray
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Escherichia coli / genetics
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Humans
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Recombinant Proteins / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
Substances
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CLIC4 protein, human
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Chloride Channels
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Recombinant Proteins