Abstract
Recombinant Escherichia coli B834 (DE3) pDB5 expressing the Rv3049c gene encoding a Baeyer-Villiger monooxygenase from Mycobacterium tuberculosis H37Rv was used for regioselective oxidations of fused bicyclic ketones. This whole-cell system represents the first recombinant Baeyer-Villiger oxidation biocatalyst that effectively resolves the racemic starting materials in this series. Within biotransformations using this organism one substrate enantiomer remains in high optical purity, while the second enantiomer is oxidized to one type of regioisomeric lactone preferably.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Biological Products / chemistry
-
Bridged Bicyclo Compounds / chemistry*
-
Catalysis
-
Escherichia coli / enzymology
-
Escherichia coli / genetics
-
Ketones / chemistry*
-
Ketones / metabolism*
-
Lactones / chemistry
-
Molecular Structure
-
Mycobacterium tuberculosis / enzymology*
-
Mycobacterium tuberculosis / genetics
-
Oxidation-Reduction
-
Oxygenases / genetics
-
Oxygenases / metabolism*
-
Recombinant Proteins / genetics
-
Recombinant Proteins / metabolism*
-
Substrate Specificity
Substances
-
Biological Products
-
Bridged Bicyclo Compounds
-
Ketones
-
Lactones
-
Recombinant Proteins
-
Oxygenases