Abstract
Early passage bovine brain capillary endothelial cells were immortalized by transfection with the plasmid pSV3 neo. Cells from one clone, SV-BEC, expressed nuclear SV 40 large T antigen, displayed a contact-inhibited and anchorage-dependent proliferation, and a high sensitivity to the addition of exogenous basic fibroblast growth factor. SV-BEC cells are morphologically unaltered and express typical markers of endothelial cells: Factor VIII-related antigen, angiotensin-converting enzyme and Griffonia simplicifolia agglutinin binding site. Endothelium like immunoreactivity was detected in the conditioned medium from these cells. Moreover, SV-BECs present numerous intercellular tight junctions characteristic of the blood-brain barrier and possess functional beta 1- and beta 2-adrenergic receptors, as observed on isolated bovine brain capillaries.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenergic beta-Antagonists / pharmacology
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Animals
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Antigens, Polyomavirus Transforming / genetics
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Biomarkers
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Blood-Brain Barrier*
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Capillaries
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Cattle
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Cell Division / drug effects
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Cell Line, Transformed
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Cells, Cultured
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Cerebrovascular Circulation*
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Culture Techniques / methods
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Cyclic AMP / metabolism
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Endothelins / analysis
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Endothelium, Vascular / cytology*
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / physiology
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Fibroblast Growth Factor 2 / pharmacology
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Intercellular Junctions / physiology
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Intercellular Junctions / ultrastructure
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Isoproterenol / pharmacology
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Microscopy, Electron
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Plasmids
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Propanolamines / metabolism
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Radioimmunoassay
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Receptors, Adrenergic, beta / drug effects
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Receptors, Adrenergic, beta / metabolism
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Simian virus 40 / genetics*
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Transfection
Substances
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Adrenergic beta-Antagonists
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Antigens, Polyomavirus Transforming
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Biomarkers
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Endothelins
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Propanolamines
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Receptors, Adrenergic, beta
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Fibroblast Growth Factor 2
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Cyclic AMP
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Isoproterenol
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CGP 12177