Abstract
In this study, we investigated the expression and function of thymosinalpha1 (Thyalpha1) in human pancreatic cancer. We found that human pancreatic cancer cell lines Panc-1, Panc03.27, ASPC-1, and PL45 cells significantly over-expressed the mRNA of Thyalpha1 as compared to the normal human pancreatic ductal epithelium (HPDE) cells.. Thyalpha1 mRNA and protein levels were also over-expressed in clinical pancreatic adenocarcinoma specimens. In addition, synthetic Thyalpha1 significantly promoted Panc-1 cell proliferation and increased phosphorylation of ERK1/2 and JNK. Furthermore, Thyalpha1 increased the secretion of multiple cytokines including IL-10, IL-13, and IL-17 in Panc-1 cells. Thus, Thyalpha1 may have a new role in pancreatic cancer pathogenesis.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line, Tumor
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Cell Proliferation / drug effects*
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Cytokines / metabolism*
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Dose-Response Relationship, Drug
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Enzyme Activation / drug effects
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Gene Expression Regulation, Neoplastic
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Humans
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Immunohistochemistry
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Interleukin-10 / metabolism
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Interleukin-13 / metabolism
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Interleukin-17 / metabolism
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JNK Mitogen-Activated Protein Kinases / metabolism
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Mitogen-Activated Protein Kinase 1 / metabolism
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Mitogen-Activated Protein Kinase 3 / metabolism
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Mitogen-Activated Protein Kinases / metabolism*
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Pancreatic Neoplasms / genetics
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Pancreatic Neoplasms / metabolism
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Pancreatic Neoplasms / pathology*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Th2 Cells / metabolism
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Thymalfasin
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Thymosin / analogs & derivatives*
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Thymosin / genetics
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Thymosin / metabolism
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Thymosin / pharmacology
Substances
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Cytokines
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Interleukin-13
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Interleukin-17
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RNA, Messenger
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Interleukin-10
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Thymosin
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JNK Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases
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Thymalfasin