A hyaluronidase supercatalyst for the enzymatic polymerization to synthesize glycosaminoglycans

Chemistry. 2006 Aug 7;12(23):5962-71. doi: 10.1002/chem.200600191.

Abstract

Hyaluronidase (HAase) catalyzes multiple enzymatic polymerizations with controlling regio- and stereoselectivity perfectly. This behavior, that is, the single enzyme being effective for multireactions and retaining the enzyme catalytic specificity, is not usual, and hence, HAase is a supercatalyst. Various sugar oxazoline monomers prepared based on the concept "transition-state analogue substrate" were successfully polymerized and copolymerized with HAase catalysis, yielding natural and unnatural glycosaminoglycans.

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Catalysis
  • Glycosaminoglycans / chemical synthesis*
  • Glycosaminoglycans / chemistry
  • Hyaluronoglucosaminidase / chemistry*
  • Molecular Sequence Data
  • Stereoisomerism
  • Time Factors

Substances

  • Glycosaminoglycans
  • Hyaluronoglucosaminidase