Blinded comparison of repetitive-sequence PCR and multilocus sequence typing for genotyping methicillin-resistant Staphylococcus aureus isolates from a children's hospital in St. Louis, Missouri

J Clin Microbiol. 2006 Jun;44(6):2254-7. doi: 10.1128/JCM.00690-06.

Abstract

We performed a blinded study to compare repetitive-sequence PCR and multilocus sequence typing for genotyping hospital- and community-acquired methicillin-resistant Staphylococcus aureus (MRSA). The MRSA strains that were sequence type 8 (ST8), staphylococcal cassette chromosome mec (SCCmec) type IV, and Panton-Valentine leukocidin-positive clustered separately from those that were ST5 and SCCmec type II.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Adolescent
  • Bacterial Typing Techniques
  • Child
  • Child, Preschool
  • Community-Acquired Infections / microbiology
  • Cross Infection / microbiology
  • Genotype
  • Hospitals, Pediatric*
  • Humans
  • Methicillin Resistance*
  • Microbial Sensitivity Tests
  • Missouri
  • Polymerase Chain Reaction / methods*
  • Repetitive Sequences, Nucleic Acid / genetics*
  • Sequence Analysis, DNA*
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus / classification*
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / genetics