Detection of multidrug-resistant protein, P-glycoprotein in childhood leukaemia and lymphoma

Eur J Pediatr. 1991 Apr;150(6):416-8. doi: 10.1007/BF02093722.

Abstract

Flow cytometric detection of surface P-glycoprotein, a multidrug-resistant gene product, with a monoclonal antibody, MRK 16, was performed on cells obtained from 18 children with leukaemia and lymphoma. Of 18 patients examined, 1 with malignant lymphoma at relapse showed a significant increase in P-glycoprotein-positive cells and a strong resistance to chemotherapy. Overexpression of P-glycoprotein in a case with B-cell type malignant lymphoma was confirmed by immuno-precipitation and Northern hybridization analysis. The present study suggests that an increased expression of surface P-glycoprotein might be involved in multidrug resistance at least in a certain case of childhood leukaemia and lymphoma.

Publication types

  • Case Reports

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Adolescent
  • Antibodies, Monoclonal
  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use
  • Blotting, Northern
  • Child
  • Drug Resistance*
  • Female
  • Flow Cytometry
  • Humans
  • Leukemia / drug therapy
  • Leukemia / metabolism*
  • Leukemia, Myeloid, Acute / metabolism
  • Lymphoma, B-Cell / metabolism
  • Lymphoma, Non-Hodgkin / drug therapy
  • Lymphoma, Non-Hodgkin / metabolism*
  • Male
  • Membrane Glycoproteins / analysis*
  • Neoplasm Proteins / analysis*
  • Precipitin Tests
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / metabolism

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Antibodies, Monoclonal
  • Membrane Glycoproteins
  • Neoplasm Proteins