Polyion complex micelles for photodynamic therapy: incorporation of dendritic photosensitizer excitable at long wavelength relevant to improved tissue-penetrating property

J Control Release. 2006 Jun 12;113(1):73-9. doi: 10.1016/j.jconrel.2006.03.009. Epub 2006 May 15.

Abstract

A polymeric micelle (DPcZn/m) system, which is formed via an electrostatic interaction of anionic dendrimer phthalocyanine (DPcZn) and poly(ethylene glycol)-poly(l-lysine) block copolymers (PEG-b-PLL), was prepared for use as an effective photosensitizer for photodynamic therapy. DPcZn/m exhibited strong Q band absorption around 650 nm, a useful wavelength for high tissue penetration. Dynamic light scattering studies indicated that the DPcZn/m system has a relevant size of 50 nm for intravenous administration. Under light irradiation, either DPcZn or DPcZn/m exhibited efficient consumption of dissolved oxygen in a medium to generate reactive oxygen species and an irradiation-time-dependent increase in photocytotoxicity. The photodynamic efficacy of the DPcZn was drastically improved by the incorporation into the polymeric micelles, typically exhibiting more than two orders of magnitude higher photocytotoxicity compared with the free DPcZn at 60-min photoirradiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Culture Techniques
  • Cell Survival / radiation effects
  • Dendrimers / chemical synthesis*
  • Dendrimers / chemistry
  • Dendrimers / metabolism
  • Dendrimers / radiation effects
  • Dendrimers / toxicity
  • Dose-Response Relationship, Drug
  • HeLa Cells
  • Humans
  • Hydrogen-Ion Concentration
  • Indoles / chemical synthesis*
  • Indoles / chemistry
  • Indoles / metabolism
  • Indoles / radiation effects
  • Indoles / toxicity
  • Isoindoles
  • Light
  • Micelles*
  • Oxygen Consumption
  • Photochemotherapy*
  • Polyamines
  • Polyelectrolytes
  • Polyethylene Glycols / chemistry*
  • Polyethylene Glycols / toxicity
  • Polylysine / chemistry*
  • Polylysine / toxicity
  • Polymers
  • Reactive Oxygen Species / metabolism
  • Solubility
  • Static Electricity
  • Water / chemistry

Substances

  • Dendrimers
  • Indoles
  • Isoindoles
  • Micelles
  • Polyamines
  • Polyelectrolytes
  • Polymers
  • Reactive Oxygen Species
  • polyanions
  • polycations
  • Water
  • Polylysine
  • Polyethylene Glycols
  • phthalocyanine