Porphyrins conjugated to DNA as CD reporters of the salt-induced B to Z-DNA transition

Org Biomol Chem. 2006 May 21;4(10):1865-7. doi: 10.1039/b603409h. Epub 2006 Apr 21.

Abstract

The porphyrin chromophore incorporated at the 5'-position of an oligonucleotide allows the simultaneous detection of the B- to Z-DNA transition via the porphyrin Soret band circular dichroism exciton couplet signal around 420 nm and the oligonucleotide CD region below 300 nm, at micromolar concentrations.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Circular Dichroism / methods
  • DNA / chemistry*
  • DNA, Z-Form / chemistry*
  • Molecular Probes / chemistry
  • Nucleic Acid Conformation
  • Porphyrins / chemistry*
  • Salts

Substances

  • DNA, Z-Form
  • Molecular Probes
  • Porphyrins
  • Salts
  • DNA