Crystallization of recombinant Haemophilus influenzae e (P4) acid phosphatase

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 May 1;62(Pt 5):464-6. doi: 10.1107/S1744309106012358. Epub 2006 Apr 21.

Abstract

Haemophilus influenzae infects the upper respiratory tract of humans and can cause infections of the middle ear, sinuses and bronchi. The virulence of the pathogen is thought to involve a group of surface-localized macromolecular components that mediate interactions at the host-pathogen interface. One of these components is lipoprotein e (P4), which is a class C acid phosphatase and a potential vaccine candidate for nontypeable H. influenzae infections. This paper reports the crystallization of recombinant e (P4) and the acquisition of a 1.7 angstroms resolution native X-ray diffraction data set. The space group is P4(2)2(1)2, with unit-cell parameters a = 65.6, c = 101.4 angstroms, one protein molecule per asymmetric unit and 37% solvent content. This is the first report of the crystallization of a class C acid phosphatase.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Outer Membrane Proteins / chemistry*
  • Crystallization
  • Crystallography, X-Ray
  • Esterases / chemistry*
  • Haemophilus influenzae / enzymology*
  • Lipoproteins / chemistry*
  • Recombinant Proteins / chemistry

Substances

  • Bacterial Outer Membrane Proteins
  • Lipoproteins
  • Recombinant Proteins
  • e(P4) lipoprotein, Haemophilus influenzae
  • Esterases