Detection of abnormalities of PRV-1, TPO, and c-MPL genes detected by fluorescence in situ hybridization in essential thrombocythemia

Cancer Genet Cytogenet. 2006 May;167(1):39-42. doi: 10.1016/j.cancergencyto.2005.08.016.

Abstract

No specific diagnostic markers have been described in essential thrombocythemia (ET). PRV-1 (polycythemia rubra vera-1), TPO (thrombopoietin), and c-MPL (myeloproliferative leukemia virus oncogene) genes are candidate ET molecular markers because of their implication in the pathogenesis of ET. We have studied the status of PRV-1, TPO, and c-MPL genes in 30 ET patients by a fluorescence in situ hybridization (FISH) technique using three noncommercial, locus-specific probes for PRV-1 (BAC RP11-160A19, located at 19q13.2), TPO (BAC RP11-45NP16, located at 3q27), and c-MPL (BAC RP11-297L5, located at 1p34). FISH study showed no PRV-1, TPO, and c-MPL cytogenetic abnormalities in any of the analyzed cases. Our results suggest a lack of structural and numerical rearrangements (deletions, translocations, or amplifications) of PRV-1, TPO, and c-MPL genes in ET patients.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoantigens / genetics*
  • Case-Control Studies
  • Female
  • GPI-Linked Proteins
  • Humans
  • In Situ Hybridization, Fluorescence*
  • Iodide Peroxidase / genetics*
  • Iron-Binding Proteins / genetics*
  • Isoantigens / genetics*
  • Male
  • Membrane Glycoproteins / genetics*
  • Proto-Oncogene Proteins / genetics*
  • Receptors, Cell Surface / genetics*
  • Receptors, Cytokine / genetics*
  • Receptors, Thrombopoietin
  • Thrombocythemia, Essential / genetics*

Substances

  • Autoantigens
  • CD177 protein, human
  • GPI-Linked Proteins
  • Iron-Binding Proteins
  • Isoantigens
  • Membrane Glycoproteins
  • Proto-Oncogene Proteins
  • Receptors, Cell Surface
  • Receptors, Cytokine
  • Receptors, Thrombopoietin
  • MPL protein, human
  • TPO protein, human
  • Iodide Peroxidase