There are various techniques available for percutaneous biopsy of suspected diskitis/osteomyelitis. Our technique has evolved as our experience with percutaneous vertebroplasty has grown. By using a transpedicular approach, we angle a bone biopsy needle in an exaggerated caudocranial trajectory to allow eventual access across the disk space above. This approach permits sampling of the disk space, as well as both adjacent vertebral endplates. We describe our percutaneous modified vertebroplasty approach for biopsy of suspected diskitis/osteomyelitis.