[Recombinant non-structural 3A, 3B and 3AB proteins of foot-and-mouth disease virus: use for differentiation of vaccinated and infected cattle]

A S Iakovleva; A V Shcherbakov; A V Kan'shina; N S Mudrak; T A Fomina

[Recombinant non-structural 3A, 3B and 3AB proteins of foot-and-mouth disease virus: use for differentiation of vaccinated and infected cattle]

Mol Biol (Mosk). 2006 Jan-Feb;40(1):165-71.

[Article in Russian]

Authors

A S Iakovleva, A V Shcherbakov, A V Kan'shina, N S Mudrak, T A Fomina

PMID: 16523703

Abstract

Recombinant proteins 3A, 3B and 3AB were obtained by expression in Escherichia coli and purified by metal-chelate chromatography. The proteins were used as antigens in indirect ELISA to differentiate vaccinated and infected cattle. While testing 200 sera from cattle 3A-ELISA was more sensitive and specific than 3B- and 3AB-ELISA. Compared with "Chekit FMD-3ABC", 3A-ELISA showed the same level of specificity and higher level of sensitivity.

Publication types

English Abstract

MeSH terms

Animals
Antigens, Viral / immunology
Carrier State / diagnosis
Carrier State / veterinary*
Carrier State / virology
Cattle
Enzyme-Linked Immunosorbent Assay
Foot-and-Mouth Disease / diagnosis
Foot-and-Mouth Disease / prevention & control
Foot-and-Mouth Disease / virology*
Foot-and-Mouth Disease Virus / immunology*
Foot-and-Mouth Disease Virus / isolation & purification
Recombinant Proteins / immunology
Viral Nonstructural Proteins / immunology*
Viral Vaccines / immunology*

Substances

Antigens, Viral
Recombinant Proteins
Viral Nonstructural Proteins
Viral Vaccines