Identification of transcription complexes that contain the double bromodomain protein Brd2 and chromatin remodeling machines

J Proteome Res. 2006 Mar;5(3):502-11. doi: 10.1021/pr050430u.

Abstract

We use affinity purification of the double bromodomain protein Brd2 to isolate a multicomponent nuclear complex from cultured cells, and apply mass spectrometry/proteomics methods to identify the participants. We then confirm by immunoblot several transcription co-activators and co-repressors, proteins of the Swi/Snf chromatin remodeling complex, which regulate transcription control of cyclin A. This multiprotein complex is likely to contribute to cell cycle control and play a role in proliferation and cancer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / physiology
  • Cell Line
  • Chromatin Assembly and Disassembly* / genetics
  • Cyclin A / biosynthesis
  • Cyclin A / genetics
  • Cyclin A / metabolism
  • Humans
  • Mass Spectrometry
  • Molecular Sequence Data
  • Multiprotein Complexes / chemistry*
  • Multiprotein Complexes / genetics*
  • Multiprotein Complexes / metabolism
  • Protein Serine-Threonine Kinases / chemistry*
  • Protein Serine-Threonine Kinases / genetics*
  • Protein Serine-Threonine Kinases / physiology
  • Protein Structure, Tertiary / genetics
  • Transcription Factors
  • Transcription, Genetic*

Substances

  • BRD2 protein, human
  • Cell Cycle Proteins
  • Cyclin A
  • Multiprotein Complexes
  • Transcription Factors
  • Protein Serine-Threonine Kinases