Crystallization and preliminary crystallographic analysis of endo-1,3-beta-glucanase from Arthrobacter sp

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt 1):68-70. doi: 10.1107/S1744309104027915. Epub 2004 Nov 9.

Abstract

Endo-1,3-beta-glucanases hydrolyze internal 1,3-beta-glucosyl linkages. The endo-1,3-beta-glucanase from Arthrobacter sp. was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group P4(1), with unit-cell parameters a = 71.31, c = 60.07 A, and contained one molecule per asymmetric unit. The Matthews coefficient (VM) and the solvent content were 2.35 A3 Da(-1) and 47.63%, respectively. Diffraction data were collected to a resolution of 1.66 A at SPring-8 using a MAR CCD area detector and gave a data set with an overall Rmerge of 5.4% and a completeness of 99.4%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthrobacter / enzymology*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / isolation & purification
  • Chromatography, Gel
  • Chromatography, Ion Exchange
  • Crystallography, X-Ray
  • Glycoside Hydrolases / chemistry*
  • Glycoside Hydrolases / isolation & purification
  • Models, Molecular
  • Protein Structure, Secondary

Substances

  • Bacterial Proteins
  • Glycoside Hydrolases
  • licheninase