Microinjection of the L-type calcium channel antagonist diltiazem into the ventral nucleus accumbens shell facilitates cocaine-induced conditioned place preferences

Biol Psychiatry. 2006 Jun 15;59(12):1236-9. doi: 10.1016/j.biopsych.2005.09.024. Epub 2006 Feb 3.

Abstract

Background: Calcium (Ca2+) influx within the nucleus accumbens shell (NASh) can influence brain reward processes. We found previously that rats self-administer NMDA receptor antagonists (which block Ca2+ influx through NMDA receptors) into the NASh. We also found that manipulations which increase expression of Ca2+-permeable AMPA receptors within this region make cocaine aversive. Here we examined if Ca2+ influx via L-type Ca2+ channels within the NASh would influence cocaine reward.

Methods: Rats received bilateral microinjections of the L-type Ca2+ channel antagonist diltiazem into the ventral NASh prior to place conditioning with systemic cocaine.

Results: Microinjections of diltiazem (10 nmol/hemisphere) into the ventral NASh facilitated the ability of a sub-threshold dose of cocaine (5.0 mg/kg) to establish place preferences, but did not affect place conditioning on their own (5.0-40 nmol/hemisphere). Microinjections into more dorsal regions had no effects.

Conclusions: Blockade of Ca2+ influx through L-type channels Ca2+ within the ventral NASh increases cocaine reward.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Anesthetics, Local / administration & dosage
  • Animals
  • Calcium Channel Blockers / pharmacology*
  • Calcium Channels, L-Type / drug effects
  • Calcium Channels, L-Type / metabolism
  • Choice Behavior / drug effects*
  • Cocaine / administration & dosage*
  • Conditioning, Operant / drug effects*
  • Diltiazem / pharmacology*
  • Dose-Response Relationship, Drug
  • Male
  • Microinjections
  • Nucleus Accumbens / drug effects*
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Anesthetics, Local
  • Calcium Channel Blockers
  • Calcium Channels, L-Type
  • Diltiazem
  • Cocaine