Abstract
Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene (spa, 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.
Publication types
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Evaluation Study
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Multicenter Study
MeSH terms
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Bacterial Typing Techniques* / methods
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Bacterial Typing Techniques* / statistics & numerical data
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DNA, Bacterial / analysis
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Humans
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Laboratories
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Observer Variation
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Reproducibility of Results
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Sequence Analysis, DNA* / methods
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Sequence Analysis, DNA* / statistics & numerical data
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Software
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Staphylococcal Infections / microbiology
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Staphylococcal Protein A / genetics*
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Staphylococcus aureus / classification*
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Staphylococcus aureus / genetics
Substances
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DNA, Bacterial
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Staphylococcal Protein A