Expression of C-terminal truncated and full-length Babesia bigemina rhoptry-associated protein 1 and their potential use in enzyme-linked immunosorbent assay

Vet Parasitol. 2006 Apr 15;137(1-2):28-35. doi: 10.1016/j.vetpar.2005.12.025. Epub 2006 Jan 27.

Abstract

Recombinant antigen-based enzyme-linked immunosorbent assay (ELISA) was developed for the serological diagnosis of Babesia bigemina infection by using a full-length B. bigemina rhoptry-associated protein 1 (rRAP-1) and the truncated C-terminal RAP-1 (rRAP-1/CT). While the rRAP-1 showed cross reactivity between B. bigemina- and Babesia bovis-infected bovine sera, the rRAP-1/CT was highly specific to B. bigemina-infected bovine sera and proved useful in the detection of sequential sera collected from an experimentally infected cow during the acute and latent infection. The high yield of soluble rRAP-1/CT and its diagnostic specificity demonstrate its potential in the diagnosis of B. bigemina infection. Its usefulness for epidemiological investigation is currently being evaluated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Protozoan*
  • Babesia / immunology*
  • Babesiosis / diagnosis
  • Babesiosis / parasitology
  • Babesiosis / veterinary*
  • Cattle
  • Cattle Diseases / diagnosis*
  • Cattle Diseases / parasitology*
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Fluorescent Antibody Technique, Indirect / methods
  • Fluorescent Antibody Technique, Indirect / veterinary
  • Gene Expression
  • Mice
  • Protozoan Proteins / genetics
  • Protozoan Proteins / immunology*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Sensitivity and Specificity

Substances

  • Antigens, Protozoan
  • Protozoan Proteins
  • Recombinant Proteins
  • rhoptry-associated protein-1, Babesia