Plane-wave fluorescence tomography with adaptive finite elements

Opt Lett. 2006 Jan 15;31(2):193-5. doi: 10.1364/ol.31.000193.

Abstract

We present three-dimensional fluorescence yield tomography of a tissue phantom in a noncontact reflectance imaging setup. The method employs planar illumination with modulated light and frequency domain fluorescence measurements made on the illumination plane. An adaptive finite-element algorithm is used to handle the ill-posed and computationally demanding inverse image reconstruction problem. Tomographic images of fluorescent targets buried at 1-2 cm depths from the illumination surface demonstrate the feasibility of fluorescence tomography from reflectance tomography in clinically relevant tissue volumes.

Publication types

  • Evaluation Study

MeSH terms

  • Algorithms*
  • Computer Simulation
  • Finite Element Analysis
  • Image Enhancement / methods
  • Image Interpretation, Computer-Assisted / methods*
  • Imaging, Three-Dimensional / methods*
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods*
  • Models, Biological*
  • Phantoms, Imaging
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Tomography, Optical / instrumentation
  • Tomography, Optical / methods*