Detection of human MCP-4/CCL13 isoforms by SELDI immunoaffinity capture

Leonardo Rossi; Ramy Moharram; Brian M Martin; Richard L White; Monica C Panelli

doi:10.1186/1479-5876-4-5

Detection of human MCP-4/CCL13 isoforms by SELDI immunoaffinity capture

J Transl Med. 2006 Jan 24:4:5. doi: 10.1186/1479-5876-4-5.

Authors

Leonardo Rossi¹, Ramy Moharram, Brian M Martin, Richard L White, Monica C Panelli

Affiliation

¹ Department of Human Morphology and Applied Biology, University of Pisa, 56126 Pisa, Italy. leoros@biomed.unipi.it

Abstract

Monocyte Chemoattractant Proteins 4 (MCP-4/CCL13) is a member of a distinct, structurally-related subclass of CC chemokines mainly involved in recruitment of eosinphils to inflammatory sites. Recent evidence demonstrates that serum level of this protein strongly increases following high dose IL-2 immunotherapy. The physiological form of human MCP-4/CCL13 has yet to be purified. Therefore, the primary structure of the biologically relevant (mature) form has not been established. By using SELDI immunoaffinity capture technology we describe two mature isoforms both present in serum before and after high-dose IL-2 immunotherapy.